Cell Structure
Light microscope

Transmission Electron Microscope
- Uses particle beam of electrons which is diffracted as it passes through the specimen
- Gives more detailed microscopic images
EXPERIMENT
To prepare and examine one animal and one plant cell, unstained and stained using a light microscope.
1) Set up microscope
2) Swab inside of cheek with cotton bud, place on slide, cover drop of water
3) When placing cover seal, place down slowly from a 45 degree angle using seeker needle to avoid air bubbles.
4) Examine slide using microscope
5) For stained cover slide with methylene blue dye instead of water and allow stand for a minute, remove any excess dye with absorbent paper.
6) When analysing plant cells cut plant e.g. celery thinly so light can pass through.
Result

Stained Unstained
CELL STRUCTURE AND FUNCTION
Organelle: Special membrane bound structure within the cell that has a specific function.
Cell Membrane
- Arranged in a phospholipid bilayer
- Boundary of the cell, holds the contents in
-Regulates what enters and leaves the cell; selectively permeable
Ribosome
- Made of RNA
- Makes proteins needed for cells; protein synthesis
Lysosome
- Contains enzymes
- Breaks down cell debris, viruses, bacteria, worn out organelles (cleaners)
Cytoplasm
- Consists of cytosol
- Functions as a medium for all chemical processes
Mitochondria
- Functions in respiration; powerhouse of the cell
- Has its own DNA(plasmid) so it can replicate by itself
Nucleus
Nucleolus
- May be more than 1
- Makes RNA(ribonucleic acid), which is then transported to the cytoplasm
Nucleoplasm
- liquid part of nucleus, similar function as cytoplasm
DNA
- Deoxyribose Nucleic Acid
- Located in the chromosomes
- Contains all the genetic code needed to make proteins
Nuclear Envelope
- Double membrane separating it from the rest of the cells contents
- Contains nuclear pores which are small holes that allows chemicals to pass in and out of the nucleus

Plant Cell
Cell Wall
- Strong and rigid to give plant strength and shape
Chloroplasts
- Functions in photosynthesis using water, CO2 and sunlight
- Have their own DNA and can replicate independently
Middle Lamella
- Cement which binds cell walls together maintaining the structure of the plant
Vacuole
- Large fluid filled sac
- Functions in storing water, food and waste
- Maintains shape of the cell
Prokaryotic Cell
- No membrane bound nucleus or membrane bound organelles, chemicals to complete their processes are present
- e.g. Bacteria
Eukaryotic Cell
- Has a membrane bound nucleus and membrane bound organelles
- Larger cells to separate all the cell processes
Light microscope

Transmission Electron Microscope
- Uses particle beam of electrons which is diffracted as it passes through the specimen
- Gives more detailed microscopic images
EXPERIMENT
To prepare and examine one animal and one plant cell, unstained and stained using a light microscope.
1) Set up microscope
2) Swab inside of cheek with cotton bud, place on slide, cover drop of water
3) When placing cover seal, place down slowly from a 45 degree angle using seeker needle to avoid air bubbles.
4) Examine slide using microscope
5) For stained cover slide with methylene blue dye instead of water and allow stand for a minute, remove any excess dye with absorbent paper.
6) When analysing plant cells cut plant e.g. celery thinly so light can pass through.
Result

Stained Unstained
CELL STRUCTURE AND FUNCTION

Cell Membrane
- Arranged in a phospholipid bilayer
- Boundary of the cell, holds the contents in
-Regulates what enters and leaves the cell; selectively permeable
Ribosome
- Made of RNA
- Makes proteins needed for cells; protein synthesis
Lysosome
- Contains enzymes
- Breaks down cell debris, viruses, bacteria, worn out organelles (cleaners)
Cytoplasm
- Consists of cytosol
- Functions as a medium for all chemical processes
Mitochondria
- Functions in respiration; powerhouse of the cell
- Has its own DNA(plasmid) so it can replicate by itself
Nucleus
Nucleolus
- May be more than 1
- Makes RNA(ribonucleic acid), which is then transported to the cytoplasm
Nucleoplasm
- liquid part of nucleus, similar function as cytoplasm
DNA
- Deoxyribose Nucleic Acid
- Located in the chromosomes
- Contains all the genetic code needed to make proteins
Nuclear Envelope
- Double membrane separating it from the rest of the cells contents
- Contains nuclear pores which are small holes that allows chemicals to pass in and out of the nucleus

Plant Cell
Cell Wall
- Strong and rigid to give plant strength and shape
Chloroplasts
- Functions in photosynthesis using water, CO2 and sunlight
- Have their own DNA and can replicate independently
Middle Lamella
- Cement which binds cell walls together maintaining the structure of the plant
Vacuole
- Large fluid filled sac
- Functions in storing water, food and waste
- Maintains shape of the cell
Prokaryotic Cell
- No membrane bound nucleus or membrane bound organelles, chemicals to complete their processes are present
- e.g. Bacteria
Eukaryotic Cell
- Has a membrane bound nucleus and membrane bound organelles
- Larger cells to separate all the cell processes
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